ALT A 20-year-old female with lower back rash (circular lesions) & burning pain for 1 week, after being stepped on by a horse. The rash consisted of multiple circular lesions with honey-colored discharge that were slightly scabbed over. An aerobic/anaerobic culture was ordered. A colony Gram stain was performed, which demonstrated septate hyphae, arthroconidia and chlamydospores. A colony tape prep with Lactophenol Aniline Blue demonstrated these same structures plus macroconidia. Subsequently, MALDI-TOF MS was performed using an alternative library, which led to this surprising organism identification. What's the diagnosis? A. Nocardia. B. Dermatophyte. C. Fusarium. D. Blastomyces.

ALT Dose-dependent, two-phase colonization pattern of C. jejuni in the ferret gut.

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ALT Overview of clinical specimens, library workflows, and read composition. (A) Distribution of 96 clinical specimens across five sample types.

ALT Representative student artifacts from Group 1 illustrating metaphor-based mechanistic reasoning.

ALT Growth on minimal media at 37°C. Strains 7, NR70, and NR71 were streaked on minimal plates containing either 0.4% glucose or 0.4% succinate as the sole carbon and energy source. Left (glucose minimal medium): strains 70 and 71 were streaked out and incubated at 37°C for 2 days, at which time strain 7 was streaked out and the plate incubated for another 2 days. Right (succinate minimal medium): strains NR70 and NR71 were streaked out and incubated at 37°C for 4 days, at which time strain 7 was streaked out and the plate incubated for an additional 2 days at 37°C.

ALT Elevated Ca2+ increases adherence of P. aeruginosa to epithelial cells. A549 (A) and CuFi-5 (B) cells were infected with PAO1 and FRD1 for 2 h at low (~0.4 mM) or high Ca2+ (5 mM) and visualized by immunofluorescence microscopy. P. aeruginosa-infected cells were stained with an anti-whole cell primary antibody followed by fluorescent secondary antibody (green), actin cytoskeleton was stained with phalloidin (red), and cell nuclei were stained with DAPI (blue). Images are representative of three independent biological replicates. Scale bar, 10 μm.

ALT KOH wet mounts of nail tissue containing dermatophyte hyphae with arthroconidia, stained with Parker Ink (A and B), viewed under a light microscope, and calcofluor white (C), viewed using a fluorescence microscope with ultra-violet light. Images in panels A and B: Sarah Kidd, School of Biological Sciences, Adelaide University.

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ALT Differential response of chickens to infections.