ALT AS2 and TOP1α repress the KRP5/ICK3 gene to promote leaf expansion. (D) Gross morphology of as2-1 top1α-1 and as2-1 top1α-1 krp5-1. Scale bars: 10 mm (5 mm in inset of as2-1 top1α-1). Plants photographed at 22 days after sowing. (E) Frequencies of phenotypes of leaves and plant lethality at 22 DAS.

ALT Cover: A wild, female Paryhale specimen stained with phalloidin and DAPI and then imaged by light-sheet microscopy and processed using Zen software at the 2025 Quintay International Course on Developmental Biology. Students from the course collected the specimen in Quintay Bay, Chile. Natália Heloísa de Oliveira, Fernanda Salce and Federica Salatiello captured the image, which won an image competition on the Node.

ALT Enhanced YAP activity in vgll4b mutants increases the contribution of midline progenitors to the notochord. (A) Scheme showing the KikGR photoconversion strategy. Red dashed box highlights the photoconverted region. Red bracket indicates the displacement distance between front of the photoconverted notochord and the front of the fotoconverted floor plate. (B) Confocal images of photoconverted control and mutant embryos at different time points. Scale bars: 100 μm. 0 h post-photoconversion (hpc) corresponds to 18 hpf. Dashed lines highlight the notochord. Yellow brackets indicate displacement distance.