The Muir lab investigates the physiochemical basis of protein function in complex systems of biomedical interest.
Princeton, NJ
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Check out our latest efforts published in @ChemicalScience on characterizing and engineering the NrdJ-1 intein for protein biochemistry and chemical biology applications! Congrats to @XuanjiaYe, Josh, Christian, @thegoodnush and Juner! @PrincetonChem
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With the start of the new academic year comes several farewells from our lab, as senior members of the lab graduate. First off, congratulations to our “transposein” Dr Yi Hua, who is heading off to @Stanford for a postdoc with @aliceyting
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Congratulations to Tom for receiving the Ralph Hirschmann award in Peptide Chemistry! @PrincetonChem
cen.acs.org/people/awards/AC…
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Congratulations to Christian, Girum, Nick, Xuanjia, and Yutong!
Link: nature.com/articles/s41586-0…
@PrincetonChem @PrincetonInnov
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What if protein function could be triggered only when the right cell cues are present? With SMART, we combine split inteins and logic gating to create a programmable system for cell-specific protein ligation, thus opening new doors for synthetic biology and cell targeting.
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Congratulations to our trailblazing leader!
Proud to announce that Tom Muir has been awarded the 2025 Bristol Myers Squibb Award in Enzyme Chemistry and Chemical Biology from the ACS Division of Biochemistry and Chemical Biology.
Congratulations Tom! Announcement: bit.ly/4dBxNtM
ALT Black-and-white image of Tom Muir standing on the staircase at Frick Lab. (Photo Jon Darmon)
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Congratulations to our second years Alan and Chuqi for passing their general exams and officially becoming PhD candidates!
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That’s a wrap for our 3rd year seminars, who all did a fantastic job presenting updates on their research progress!
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Check out our latest review in Accounts of Chemical Research showcasing how integrating chemical biology, biochemistry, and genetics tools can enable deep interrogation of biological pathways involved in bacterial quorum sensing. Congrats @StevenPBodine!
pubs.acs.org/doi/full/10.102…
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Thank you to @ScienceMagazine and @Nature for highlighting our protein transposition technology
Science perspective:
science.org/doi/10.1126/scie…
Nature perspective:
nature.com/articles/d41586-0…
The final version of our protein transposition manuscript is now published in @ScienceMagazine
science.org/doi/10.1126/scie…
We’ve added a few new things since our preprint /1
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We are also able to show that this protein editing method works in live cells with high fidelity. We are excited to see where we can push the limits of this system in future studies of proteins in native biological contexts. /3
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Our reviewers wanted to know if our transposition system was truly coordinated, and by measuring the rates of intein splicing, we find that with the optimized intein pair, transposition is indeed coordinated. /2
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The final version of our protein transposition manuscript is now published in @ScienceMagazine
science.org/doi/10.1126/scie…
We’ve added a few new things since our preprint /1
Ever wanted to swap out an entire internal region of a folded protein for functional biochemical studies? Check out our preprint that has been 5 years in the making, in which we establish a protein editing platform via protein transposition, enabling the insertion of functional cargo or PTMs into internal folded protein regions. #proteinengineering #proteinediting
biorxiv.org/content/10.1101/…
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Check out our new review in @jbiolchem by @esmathegazi on the recent advances in the synthesis of designer chromatin and its application towards the study of chromatin biochemistry and biology
#designerchromatin #epigenetics #PTMs @PrincetonChem @PrincetonMolBio
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In particular, we use CAGE to study the dynamics of phosphorylation events, which enable us to identify and target kinases involved in these oncofusion disease pathways!
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Our CAGE system allows us to post-translationally turn on the activity of oncogenic proteins from inactive fragments and to study their downstream signaling events with temporal control.
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Check out our latest paper in @nchembio, where we developed our CAGE split intein platform to study signaling events using proteomic profiling. This enables insights into the differential molecular connections of fusion oncogenes. Congrats to @GihoonLee1!
nature.com/articles/s41589-0…
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This work was accomplished by a talented team led by grad student Yi Hua and postdoc Nick Tay @malayoman. Many important contributions by Xuanjia Ye, @jeremyaowen, @Hengyuan_Liu03, and especially @rtho5616 for initiating the project back during his @MuirLab days
@PrincetonChem
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