1/ Hot off the presses: exciting work with @svoboda314, @RPancholi19, @TinaVoelcker, @FOlafsdottir, Jason Wittenbach, and @thefreemanlab. We show that recurrent circuitry in cortical L2/3 mediates representation-specific amplification. nature.com/articles/s41586-0…
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2/ Longstanding theoretical and experimental work argues that recurrent excitatory circuitry in cortex mediates amplification; we begin by building a model that confirms this intuition:
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3/ The model makes a very simple prediction: if elevated recurrence is present in a subnetwork, the network should be highly sensitive to removing a handful (20 neurons of 200) of its members - response in such networks will decline.
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4/ We then use cellular-resolution ablation in mice following volumetric imaging to see if touch and whisking representations in barrel cortex show this decline
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5/ touch, but not whisking networks, show decline in response among spared neurons:
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6/ Ablation effect depends on how much of the touch representation we degrade (black: silent; green: whisking; blue: touch)

Mar 4, 2020 · 5:41 PM UTC

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7/ Crucially, this method is much simpler than 2P optogenetics - you can take your sweet time targeting the neurons, and they can be very widely distributed in space. And anyone with a conventional 2P imaging setup can do this experiment!
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