Wiep Klaas Smits@SmitsLab

Assoc. prof. @LUMC_Leiden/CMAT. Cdiff, DNA replication, AMR, microbiome, anaerobes. Clostpath SC. Education officer ESGCD. Also: @smitslab.bsky.social

 Leiden, Nederland
lumc.nl/smits
Joined August 2015
340 Photos and videos
[🔔] Publication alert! The CloDiCo trial is now published in @NatureComms @NaturePortfolio (Editor's choice!). This is the work of an amazing team, and led by PhD student Annefleur Hensen. Check out this thread [👇] for a summary of the study. nature.com/articles/s41467-0… 1/10

Experimental human colonisation with non-toxigenic Clostridioides difficile: a placebo-controlled...

Nature Communications - Experimental human colonisation with non-toxigenic Clostridioides difficile shows that antibiotic-mediated microbiota disruption, particularly prolonged treatment, increases...

nature.com
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Activity-based profiling of bacterial RNA-modifying enzymes reveals species-specific 5-methyluridine modification in Bacillus subtilis 23S rRNA | PNAS pnas.org/doi/10.1073/pnas.26…

Activity-based profiling of bacterial RNA-modifying enzymes reveals species-specific 5-methylurid...

RNA modifications play an important role in biological processes. Mapping the diversity of RNA chemistry and studying the biological function of in...

pnas.org
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The underappreciated role of rifampin in shaping the epidemiology of RT027-like Clostridioides difficile lineages academic.oup.com/jac/article…

The underappreciated role of rifampin in shaping the epidemiology of RT027-like Clostridioides...

Abstract. The global emergence and dissemination of epidemic Clostridioides difficile ribotype 027 (RT027) and related lineages illustrate the role of anti

academic.oup.com
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The impact of TcdB conformational dynamics on vaccination against Clostridioides difficile: dx.plos.org/10.1371/journal.…

The impact of TcdB conformational dynamics on vaccination against Clostridioides difficile

Author summary Clostridioides difficile is a major cause of severe gastrointestinal infections. Much of the damage caused during infection is driven by the large multidomain toxin, TcdB, making it a...

journals.plos.org
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Type 2 immunity in Clostridioides difficile infection sciencedirect.com/science/ar…
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The impact of fecal microbiota transplantation on host immunity and epithelial restoration following Clostridioides difficile infection sciencedirect.com/science/ar…
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Wiep Klaas Smits retweeted
Akos Nyerges@AkosNyerges
Sep 19
Proud and excited to share our first preprint 🚀 in which we • create a high-fitness firewalled organism, • that stably colonizes the mouse gut for >100 days, • resists all tested phages in the gut, • and blocks horizontal gene transfer. + Chimera genomes to tailor synthetic
Firewalled synthetic commensal blocks horizontal gene transfer in the gut

ALT Firewalled synthetic commensal blocks horizontal gene transfer in the gut

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Clostridioides difficile couples responses to host nutritional immunity and a commensal-derived antibiotic to enable growth in the gut | Science Signaling science.org/doi/10.1126/scis…

Clostridioides difficile couples responses to host nutritional immunity and a commensal-derived...

A coordinated response to low iron and bacitracin enables Clostridioides difficile to survive in the gut.

science.org
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Wiep Klaas Smits retweeted
Daniel Bauer@danielevanbauer
Sep 16
Inspired by Gibson cloning, we describe prime assembly, now published, for in cellulo targeted integration of gene-sized DNA fragments using prime editing. nature.com/articles/s41586-0…

Targeted genomic integration and rearrangement using prime assembly

Nature - Prime assembly enables RNA-guided integration of medium-to-large DNA sequences in human cells without requiring double-strand breaks or cell cycle progression, and supports exon recoding,...

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Wiep Klaas Smits retweeted
Tominaga K. (tomiken)@pacyc1841
Sep 17
Bacterial stress responses lower mRNA–protein level correlations | PNAS pnas.org/doi/abs/10.1073/pna…

Bacterial stress responses lower mRNA–protein level correlations | PNAS

Diverse bacterial pathogens have evolved complex regulatory mechanisms to adapt to various environmental stresses during infection. The uncertainty...

pnas.org
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Wiep Klaas Smits retweeted
EMBL@embl
Sep 15
Scientists have determined the highest-resolution protein structure obtained to date. Using advanced X-ray crystallography at EMBL Hamburg's P14 beamline, the team solved the structure of an archaeal protein at an astonishing 0.43 Ångström resolution. embl.org/news/science-techno…
A valence electron deformation map of archaeal rubredoxin at 0.43 Ångström resolution

ALT A valence electron deformation map of archaeal rubredoxin at 0.43 Ångström resolution

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It's a wrap! #ICDS2026 has come to an end. As usual, flawless execution, great crowd. There will be another ICDS in two years - but not organised by Maja Rupnik anymore. Lots of respect and appreciation for what she has done over the past decades for the #cdiff community.
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Wiep Klaas Smits retweeted
Basler Lab@Basler_Lab
Sep 9
In our new paper, we uncover how Burkholderia switches from antibacterial T6SS-1 to eukaryotic-targeting T6SS-5. Surprisingly, actively turning off T6SS-1 assembly prevents mitochondrial damage and apoptosis. Congrats @christy_chc and the team! link.springer.com/article/10…
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How is your day going so far? My trip to #ICDS2026 starts with an overbooked flight resulting in me arriving half a day later than expected (if all goes "well") after being rebooked... #mondayblues #flyingblue @KLM
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but hey, at least I got a lunch voucher that will buy me exactly 1 sandwich...
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This is a great study by @joeyzacks et al on "asymptomatic" carriage of Cdiff in infants. Must read! Early-life colonization with Clostridioides difficile remodels the developing gut | Science science.org/doi/10.1126/scie…

Early-life colonization with Clostridioides difficile remodels the developing gut

Clostridioides difficile causes severe disease in adults but commonly colonizes infants asymptomatically. The consequences of early-life colonization on host development remain unknown. In a neonatal...

science.org
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Effects of sexual dimorphism and estrous cycle on murine Clostridioides difficile infection | Infection and Immunity journals.asm.org/doi/10.1128…

Effects of sexual dimorphism and estrous cycle on murine Clostridioides difficile infection |...

Clostridioides difficile infection (CDI) is responsible for approximately 25% of all antibiotic-associated diarrhea (1, 2). In the United States, over 223,900 CDI cases occur annually, with a...

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Multi-strain Clostridioides difficile infection: Increased detection and clinical implications. sciencedirect.com/science/ar…
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Clinical failure of an extended-pulsed fidaxomicin regimen associated with emergence of a Clostridioides difficile isolate with reduced fidaxomicin susceptibility in an elderly man | Antimicrobial Agents and Chemotherapy journals.asm.org/doi/10.1128…

Clinical failure of an extended-pulsed fidaxomicin regimen associated with emergence of a Clostri...

Fidaxomicin is a macrocyclic antibiotic recommended as the preferred antimicrobial treatment for Clostridioides difficile infection (CDI) when available (1, 2). Fidaxomicin is as effective as oral...

journals.asm.org
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AGA Clinical Practice Update on Management of Clostridioides difficile Infection in Adults: Expert Review sciencedirect.com/science/ar…
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Very interesting! Novel surveillance design exploiting network analysis for efficient, rapid detection of emerging Clostridioides difficile strains nature.com/articles/s41467-0…

Novel surveillance design exploiting network analysis for efficient, rapid detection of emerging...

Nature Communications - Using network analysis for inter-hospital spread and algorithmically optimising sentinel site selection, the authors design a surveillance system to rapidly detect emerging...

nature.com
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