Filippo Caschera
@Stjle

Synthetic Biology, Artificial Cells, Amphiphile Systems, Machine Learning, Cell-Free Protein Synthesis: Italy, Denmark, Japan, USA, France, Germany, UK.

 Rome, Lazio
scholar.google.co.uk/citatio…
Joined April 2010
25 Photos and videos
Pleased to share my latest review on Cell-Free Protein Synthesis #CFPS and #AI/ML for accelerating drug discovery sciencedirect.com/science/ar… #CellFree #SynBio
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Olivier Borkowski@O_Borkowski
Sep 25
Cell-free Engineering of LPST153 Bacteriophage Receptor-Binding Proteins Enables Tunable Host-Range Modulation doi.org/10.1021/acssynbio.6c…
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Cell-free systems enable mechanistic characterization of genetically encoded RNA strand exchange circuits for programmable protein expression biorxiv.org/content/10.1101/…

Cell-free systems enable mechanistic characterization of genetically encoded RNA strand exchange...

Nucleic acid circuits are powerful tools for programming biology, but the design principles for operating these circuits in complex cellular environments remain poorly understood compared to simple...

biorxiv.org
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Cell-free membrane systems reveal electrostatic control of ion transport in TMEM165 and disease-associated CaCA2 variants biorxiv.org/content/10.64898…

Cell-free membrane systems reveal electrostatic control of ion transport in TMEM165 and disease-a...

Ion transport across intracellular membranes is essential for cellular homeostasis, yet organellar transport mechanisms remain difficult to resolve in native cells. Here, we establish a cell-free...

biorxiv.org
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Cell-free systems as complementary Test layers for protein engineering in biofoundry workflows sciencedirect.com/science/ar…
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Olivier Borkowski@O_Borkowski
Sep 15
Engineered Orthogonal Translation Systems from Metagenomic Libraries Expand the Genetic Code doi.org/10.1021/acssynbio.6c…
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visualize.jove.com/42729265-…

A liquid handling platform for standardized quantification of cell-free enzymatic activity encoded...

Rapidly characterize antimicrobial resistance genes (ARGs) with a new semi-automated workflow. Essential for combating AMR and developing new antibiotics.

visualize.jove.com
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A High-Throughput Assay to Identify Specific Nascent Chain Inhibitors biorxiv.org/content/10.64898…

A High-Throughput Assay to Identify Specific Nascent Chain Inhibitors

“Undruggable” proteins without surface-accessible binding sites pose significant challenges to target-based drug discovery. Innovative approaches are needed to tackle these proteins. One promising...

biorxiv.org
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Olivier Borkowski@O_Borkowski
Sep 7
A Two-step Gene Expression Method for In Vitro Evolution of Translational Proteins doi.org/10.1021/acssynbio.6c…
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Ichihashi Lab Komaba@KomabaLab
Sep 5
櫻井・庄司・市橋でやった論文が出ました。ドロプレット内反応を2段階で行うことで、翻訳タンパク質の完全in vitro進化ができるようになりました。WTより比活性の高いEF-Gも作れました pubs.acs.org/asbcd6/article/…

A Two-step Gene Expression Method for In Vitro Evolution of Translational Proteins

Abstract. Improving the reconstituted translation system is a key requirement for bottom-up synthetic biology. Here, we developed a two-step in vitro evolu

pubs.acs.org
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Autonomous Homeostatic Synthetic Cells via Self-Gating DNA Nanopores biorxiv.org/content/10.64898…

Autonomous Homeostatic Synthetic Cells via Self-Gating DNA Nanopores

Homeostasis is a fundamental hallmark of living organisms, arising from the complex interplay between biochemical reactions and regulatory feedback systems. Reconstituting such self-regulating...

biorxiv.org
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Maerkl Lab@MaerklLab
Sep 3
Jiami and Laura's paper is out now! In collaboration with the Reddy lab at ETHZ! cell.com/cell-systems/fullte…

High-throughput cell-free profiling of SARS-CoV-2 RBD variants enables rapid and quantitative in...

The team developed a high-throughput platform to rapidly measure how hundreds of viral mutation combinations affect antibody binding, generating 31,740 quantitative affinity data points. The result...

cell.com
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Functional mapping and engineering of the Sec translocon unlocked by a cell-free system | Science Advances science.org/doi/10.1126/scia…

Functional mapping and engineering of the Sec translocon unlocked by a cell-free system

Almost all membrane proteins are inserted or translocated across membranes by the universally conserved Sec translocon. Despite its central role, experimental access to Sec function has remained...

science.org
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Timing of metabolomics-driven supplementation strategies affects protein expression in E. coli-based cell-free expression systems | bioRxiv biorxiv.org/content/10.64898…

Timing of metabolomics-driven supplementation strategies affects protein expression in E. coli-ba...

While in vivo synthesis of biologic therapeutics has been broadly successful, it is limited by biological constraints of the cells and by the complexity, time, and cost of implementing the pipeline...

biorxiv.org
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liquid handling platform for standardised quantification of cell-free enzymatic activity encoded by antimicrobial resistance genes academic.oup.com/synbio/arti…

A liquid handling platform for standardized quantification of cell-free enzymatic activity encoded...

Abstract. Antimicrobial resistance (AMR) is a growing global threat to human health, and rapid methods for characterizing emerging antimicrobial resistance

academic.oup.com
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Our work is out in ACS SynBio. - More T7RNAP effective ONLY at low DNA - LESS(!) ribosome is good at low DNA - ~14nM mRNA & ~96nM mNG-GFP from 0.2pM(!) DNA input - Easy-to-use recipe for direct signal readout from microdroplets pubs.acs.org/asbcd6/article/… (with @NaohiroTerasaka lab)

Boosted Cell-Free Gene Expression for Robust Signal Readout from a Single-Copy DNA Template in...

Abstract. Cell-free gene expression in microcompartments constitutes a chassis for biotechnology and synthetic biology. Protein synthesis from low DNA conc

pubs.acs.org
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Rethinking vaccine manufacturing using cell-free systems: challenges and opportunities insights.bio/vaccine-insight…

Rethinking vaccine manufacturing using cell-free systems: challenges and opportunities

Cell-free protein synthesis (CFPS) has emerged as an attractive technology for decentralized manufacturing of proteins, offering the ability to decouple lysate production from protein synthesis,...

insights.bio
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Automated prototyping of genetic codes | Nature nature.com/articles/s41586-0…

Automated prototyping of genetic codes

Nature - A robotic, cell–free platform rapidly prototypes redesigned genetic codes, enabling the translation of proteins with reassigned codons and non-standard amino acids without altering...

nature.com
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ORB-TXTL: cell-free expression of membrane proteins on lipid bilayer-coated beads biorxiv.org/content/10.64898…

ORB-TXTL: cell-free expression of membrane proteins on lipid bilayer-coated beads

Membrane proteins achieve a remarkable range of cellular functions, yet their characterization at high throughputs remains difficult with standard reconstitution methods. Here, we develop On-bead...

biorxiv.org
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Filippo Caschera retweeted
Olivier Borkowski@O_Borkowski
Aug 24
Engineering erythrocytes into synthetic cells using cell-free gene expression doi.org/10.64898/2026.08.20.…
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Establishing Cell Free Protein Expression as the Preferred Screening Platform for AI Driven Drug Discovery pubs.acs.org/symposium/edite…

Establishing Cell Free Protein Expression as the Preferred Screening Platform for AI Driven Drug...

AbstractAdvances in artificial intelligence and machine learning (AI/ML) have transformed protein design by enabling efficient exploration of vast sequence

pubs.acs.org
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