One does not simply use the FIB, one must become the FIB.
Many thanks to @timmaitland1 and Roy MacDoland for their amazing work as we go through this installation.
attention NIH! giving David Mastronarde an extra $5M would do a lot more for the structural biology community than funding one more microscope, because his output increases the productivity of most of the cryoelectron microscopes worldwide
I'd like to propose another trend: in addition to hugging your Krios, why not carry your K3... this will allow you to show off your fancy DED and also your upper body strength (75 lbs!). Here it goes:
Came across this photo while searching for another. You can see a grid held in the "chopsticks" of a dual axis(DA)fixed holder (bottom of pic). The cartridge axis is on its way to retrieve the grid to take it back to the autoloader (top of pic). New Krios systems won't have DA