Peter Du@peterdu_
Joined October 2020
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CRISPR screens are great at telling you what a gene is doing inside your cells. But what if you're interested in what's happening to its neighbors? Today, I'm excited to share match-seq, a new method that deciphers cell-cell interactions using barcoded RNA transfer. 1/9
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Finally, using the Perturb-match dataset, we were able to predict the immune cell type that was responsible for sensitization from each perturbation. 8/9
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Thanks to @MCBassik and @Satpathology for being the perfect mentors for a project that bridges CRISPR, tumor immunology, and single-cell genomics. Read more about match-seq here biorxiv.org/content/10.64898… 9/9

In vivo intercellular CRISPR screens using viral proximity barcoding reveal regulators of tumor-i...

Cell-cell interactions shape tumor growth, immune evasion, and therapeutic response, but systematically dissecting their genetic regulators in vivo remains challenging. Existing cell-cell interaction...

biorxiv.org
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