#Jena, that’s a wrap! We’re happy to close the #FLIM MicroSpectroscopy workshop with such a positive feeling. These two days were a great opportunity to share ideas, test workflows, and continue strengthening our collaboration with the amazing @OpenUc2 team.

🔬A new study by EMBL researchers, published in Science, about the pre-ribosome assembly pathways inside intact human cells, reveals — at molecular resolution — in-cell structural snapshots and novel configurations. science.org/doi/10.1126/scie…

#lipidtime: It's a lipid universe. Today in Nature we publish the Lipid Brain Atlas, the first map of membrane lipid composition across the entire mouse brain, a layer of organisation that cell-type and transcript maps had left out. #lipidomics #neuroscience #BrainAtlas

ʙɪɴᴅᴄʀᴀꜰᴛ2 is out, and we're not waiting for the paper. The full code drops today, free for academic and industry use. We're releasing it early so you can start designing right now, and bring its full power to the current Adaptyv competition. github.com/PacesaLab/BindCra…

In our September 2026 issue - Plasmonic nanocavities, Upconverting nanoparticles for imaging, Blue-emitting QD/SiNx lasers, Self-assembled contacts, Li+ separation, Spectral biophysical cytometry, Gold nanocages against amyloids, & much more. nature.com/nnano/volumes/21/…

ProteinDPO: a protein language model for stability prediction and generation of thermostable protein sequences. @BrianHie nature.com/articles/s41592-0…

our 5th publication of the year is now out, and it comes with our shortest title yet: ONE microscopy. nature.com/articles/s41596-0… ONE pushes conventional fluorescence microscopy to the molecular scale. It allows us to directly visualize the shapes of individual proteins, resolve conformational changes of around a nanometer, and achieve nanometer to subnanometer imaging precision, while extending these capabilities from purified molecules to complex cellular and tissue samples. This Nature Protocols paper provides the complete workflow for doing this, from sample preparation and expansion to imaging and fluctuation analysis. And now there is ONEpy, our new software package that brings the analysis pipeline together and makes it substantially easier to use. Faster to set up, easier to use, and gives us a much better base for what we want to add next. and this is not all from the team this year. We currently have 3 more papers in revision, and we are preparing a 4th manuscript to submit by the end of the year. we are only getting started, and I could not ask for a more ambitious, creative and relentless team to build this with. what started with ONE as something that seemed almost impossible is now becoming a field: optical structural biology.

very exciting new preprint from Ignacio Vega Vásquez, @LabSauer, and collaborators introducing Mega-ExM. Preprint: biorxiv.org/content/10.64898… beyond the impressive expansion itself, one result stands out to me: using only ~20 nuclear pore complexes imaged with a 40× objective on a conventional confocal microscope, we could reconstruct their 3D structure at ~33 Å resolution. Commentary for several years, we have been pushing the idea that ExM does not only expand cells, organelles, and pull proteins apart, but can rather physically expand the structure of individual protein molecules. We show that in Shaib et. al., 2024. The current work now brings optical structural reconstruction into intact cells and sets up the next challenge: pushing from supramolecular assemblies to individual protein molecules in their native cellular environment. congratulations 🎉 to Ignacio, Markus and all collaborators, and thank you for letting us contribute to this work.

Today in @ScienceMagazine, we report the first functional AI-generated genomes. 🧵 science.org/doi/10.1126/scie…

1/ What could we discover if we had a binder for every protein in the human proteome? We are excited to introduce the Bindome, an open and free resource of over 300,000 designed protein binder candidates against over 8,000 human target proteins. 🧵

Congratulations to Janna Eilts, Markus Sauer @LabSauer, and the entire team on this beautiful work. By combining 8-fold double-homogenized expanded hydrogels with dSTORM, we showed that the 8 nm spacing between neighbouring tubulin molecules can be resolved in immunolabelled cells. We also revealed that the presynaptic proteins RIM and Munc13-1 are organized into remarkably small, ~44 nm ring-like structures in neurons. Thank you, Janna and Markus, for letting us contribute a small part to this work. Article online: nature.com/articles/s41467-0…

Excited to share our work developing U-STORM super-resolution microscopy, now published @NatureNano! We engineered UCNPs that blink spontaneously and indefinitely, enabling two-color U-STORM imaging with sub-ångström localization precision using a simple optical setup without special imaging buffers. nature.com/articles/s41565-0…

🔥 Published today, and just in time for summer conferences, here are your guidelines for presenting badly

My work got another @Nature News feature: From billion-fold volumetric expansion to in situ structural biology. nature.com/articles/d41586-0… The recent @yourUMG-@MIT preprint shows what is possible on purified systems. My team's next challenge is to achieve comparable molecular resolution directly inside intact cells. a brilliant piece from @ewencallaway

I'm joining OpenAI next week!🥹 The job search turned out to be really challenging but also super rewarding, so I wrote a small blog to share what I learned along the way and hopefully make the process a little less mysterious for the next person. alisawuffles.github.io/blog/…

Expansion microscopy physically enlarges the sample, but it also dilutes fluorophores, exposes them to harsh chemistry, and leaves them in a water-rich gel. Not every dye survives that. Ali Shaib and his team at the University Medical Center Göttingen use expansion microscopy combined with AI-based analysis to study synapses, cytoskeletal structures, and individual protein molecules at the nanoscale. For single-molecule imaging, photostability is not optional. The image shows microtubules imaged with STED, ExM, ExSTED, and ONE . "Expansion microscopy is brutal on fluorophores: the sample is chemically processed, enlarged, diluted, and left in a water-rich gel. STAR 635P is one of the rare dyes that still shines bright at the end of that journey." Shaib, University Medical Center Göttingen. STAR 635P enabled stable imaging of single protein molecules over 2,000–5,000 frames with minimal bleaching, and robust labeling for combined STED and expansion microscopy. #ExM #ExpansionMicroscopy #STED #SingleMoleculeImaging #STARdyes #abberior

Mitochondria directly interact with the nuclear pore complex: nature.com/articles/s41586-0…

論文をダウンロードするときに自動リネームするchrome pluginを作りました。和文にも対応しています。 chromewebstore.google.com/de…

RNA-binding protein diversity and NLS arginines regulate FUS mixing in mRNA-rich compartments: Cell Reports cell.com/cell-reports/fullte…

Hello world, meet 1,000× Expansion Microscopy. 1,000,000,000× expansion by volume! A gel that starts at a few centimeters will then expand to the volume of an Olympic swimming pool. biorxiv.org/content/10.64898… In our new bioRxiv preprint, work carried out between MIT and UMG, led by Helena Hu in collaboration with scientists from the labs of @eboyden3 Ed Boyden, Silvio Rizzoli, and myself, we present Thousandfold Expansion Microscopy. By enlarging biological specimens across multiple rounds of expansion, molecular-scale features, as small as the distances between adjacent amino acids, can be visualized with conventional optical microscopes. Democratizing super-resolution microscopy.