The Kleiner lab investigates the chemical biology of RNA modifications and their role in gene expression regulation. kleiner.princeton.edu
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KleinerLab retweeted
What drives cytoplasmic mRNA organization? We created unbiased, genome-wide maps of mesoscale RNA-RNA spatial proximity to find out - Biggest surprise was impact of encoded protein function. Fantastic work w/ @LindsayABecker @sofiquinodoz @david_a_knowles
biorxiv.org/content/10.1101/…
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KleinerLab retweeted
The final version of our protein transposition manuscript is now published in @ScienceMagazine
science.org/doi/10.1126/scie…
We’ve added a few new things since our preprint /1
Ever wanted to swap out an entire internal region of a folded protein for functional biochemical studies? Check out our preprint that has been 5 years in the making, in which we establish a protein editing platform via protein transposition, enabling the insertion of functional cargo or PTMs into internal folded protein regions. #proteinengineering #proteinediting
biorxiv.org/content/10.1101/…
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KleinerLab retweeted
In lab's #FIRSTPREPRINT, we present methods to measure nanometer organization around & between specific proteins in condensates. We uncover unexpected heterogeneity for a liquid-like phase with local meshwork spanning 10-50nm, stemming from ribosome biogenesis in the nucleolus.
Nanometer condensate organization in live cells derived from partitioning measurements biorxiv.org/content/10.1101/… #biorxiv_biophys
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We are looking for a postdoc candidate in RNA chemical biology, broadly defined. Interested applicants can email me at rkleiner@princeton.edu with their CV, research summary, and names of 3 references. kleiner.princeton.edu/
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KleinerLab retweeted
Faculty & students from @EmmanuelCollege, @CSUSBNews and @_hamptonu spent the summer with @PrincetonChem as part of the Visiting Faculty Research Partnership, working on projects that form the core of their research at home but with special focus at Frick: bit.ly/4edp5B8
ALT Faculty and undergrads from Emmanuel College, Cal State San Bernardino and Hampton College
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KleinerLab retweeted
Congratulations to Qais Jaber, awarded a two-year @EMBO Postdoctoral Fellowship supporting excellent postdoc research.
Qais will continue his work in the @KleinerLabRNA deciphering RNA modifications in pathogenic bacteria.
ALT Full-color photo of Qais standing on the staircase in Princeton Chemistry's Frick Lab.
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KleinerLab retweeted
An honor to announce our book has been published! Many thanks to Martin Pettersson, the team at Wiley, and all our amazing contributors for making this exciting project happen! onlinelibrary.wiley.com/doi/…
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KleinerLab retweeted
We are just opening the summer Visiting Faculty Research Partnership season and are thrilled to be hosting, among others, the Graham Chakafana Lab of @_HamptonU.
The lab will be doing malaria research hosted by the @KleinerLabRNA. This marks their 2nd summer @PrincetonChem!
ALT Three individuals (Professor Chakafana in the back) working in the lab at Princeton Chemistry.
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I'm very excited to share the news of my promotion. Much of the credit goes to the trainees that drove the research forward, and the invaluable mentorship that I received along the way from Michael Hecht, @davidrliu, @KapoorLab_RU, my Princeton colleagues, and many others!
We are so very pleased to announce that @PrincetonChem’s Ralph Kleiner has been promoted to Associate Professor with tenure.
Congratulations Ralph and the @KleinerLabRNA!
Story here: bit.ly/4bJ4NhZ
ALT Color, head-and-shoulders image of Ralph Kleiner against a white backgrop.
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KleinerLab retweeted
Ever wanted to incorporate ncAAs into your protein but found the efficiency to be too low? Check out our new preprint, where we turned a simple observation in protein translation to an effective quadruplet codon translation strategy!
Efficient Genetic Code Expansion Without Host Genome Modifications
ift.tt/YImKuBd
#biorxiv_synbio
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KleinerLab retweeted
"Here, we describe our efforts to illuminate RNA biology through the application of chemical tools, focusing on post-transcriptional regulatory mechanisms."
Enabling technologies from @KleinerLabRNA
pubs.acs.org/doi/10.1021/acs…
#tbt
#ACSChemicalBiology
#methods
@PrincetonChem
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KleinerLab retweeted
What a great Take Your Child to Work Day here @PrincetonChem with faculty, visitors, and kids enjoying crafts, lunch, and a campus-wide chem demo (thank you, @PrincetonPhys).
Well done Sarah and Angie! #TYCTWD24
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KleinerLab retweeted
How to pick a problem. Today in @CellCellPress. 1/53
cell.com/cell/fulltext/S0092…
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Excited to share the latest manuscript from my lab and our first in @ACSCentSci . We used 5-BrUrd-modified tRNAs as activity-based probes to study the mechanism and substrate specificity of a human dihydrouridine synthase enzyme. Congrats to Jingwei and Nathan! @PrincetonChem
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KleinerLab retweeted
New issue! 📣
Read up on detection of RNA-protein interactions, selenoneine biosynthesis, riboswitches, sirtuins, functional amyloids, and more!
pubs.acs.org/toc/bichaw/62/2…
#amyloid
#RNA
#biosynthesis
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KleinerLab retweeted
Please see the amazing work done by @_xun_sun_ , published in Nat. Chem. today! Huge thanks to all of our great collaborators, Prof. Dr. Henning Mootz, Prof. Jhih-Wei Chu, Prof. Nozomi Ando (@AndoLab), and Prof. Tamiki Komatsuzaki (@HokkaidoUnivPR)!
nature.com/articles/s41557-0…
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KleinerLab retweeted
Would you be surprised to learn that acid chlorides are not only compatible with biological research, in water mind you, but are in fact, AMAZING?
Read on to see the answer…
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Two new preprints from our lab on human tRNA dihydrouridine (D) modifications and DUS writer enzymes:
biorxiv.org/content/10.1101/…
biorxiv.org/content/10.1101/…
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In the first, we use chemoproteomic and transcriptomic methods to study human DUS proteins and globally map tRNA D sites. We then show that DUS proteins control the levels of a subset of tRNAs and codon-specific translation in a cell type-specific manner.
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