Shabaz Lab@ShabazLab

Focus on developing proteomics technologies so mostly relates to sample prep, MS and LC.

 Oxford, England
chem.ox.ac.uk/people/shabaz-…
Joined July 2020
113 Photos and videos
I am thrilled to share that our work evaluating - deep breath - UVPD, ECD, AI-ECD, sceHCD (sceCID really), and EID for glycopeptides is out now! pubs.acs.org/doi/10.1021/acs…

Evaluating the Performance of Photon- and Electron-Based Fragmentation Methods in Omnitrap-LCMS...

Abstract. To date, collision-induced dissociation and methods based on electron transfer dissociation are considered the ‘best’ approaches for the mass spe

pubs.acs.org
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The deadline (May 19th) is getting awfully close! Don't miss out. brixenproteomics.org

A HUGE THANK YOU

FEBS Advanced Lecture Course

brixenproteomics.org
Shabaz Lab@ShabazLab
Apr 19
1 month left! brixenproteomics.org
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1 month left! brixenproteomics.org
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Shabaz Lab retweeted
Cell Reports@CellReports
Apr 2
RNA binding as an emerging regulatory dimension in the type I IFN response dlvr.it/TRr3sF

RNA binding as an emerging regulatory dimension in the type I IFN response

Iselin et al. explore how the type I IFN response regulates RNA-binding activity using RNA-interactome capture and extend the RIC protocol to assess the role of phosphorylation in this process....

cell.com
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Shabaz Lab retweeted
Kermit Murray@kermitmurray
Mar 26
(JASMS) [ASAP] Investigating the Efficiency of Ultraviolet Photodissociation in Peptides Modified with N-Terminal UV-Absorbing Chromophores dlvr.it/TRjzlg (RSS) #MassSpecRSS #JASMS
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Shabaz Lab retweeted
Mathias Wilhelm@wilhelm_compms
Mar 24
Prosit-MultiFrag, a model predicting spectra for UVPD, ECD, ETciD & EID, is out 🚀 🔗 nature.com/articles/s41592-0… Great collaboration with Shabaz Mohammed & Nikita Levin. Try it via Koina koina.proteomicsdb.org and Oktoberfest: github.com/wilhelm-lab/oktob… #Proteomics #MassSpectrometry

Integration of alternative fragmentation techniques into standard LC-MS workflows using a single...

Nature Methods - An integrated mass spectrometry platform enabling automated collision-, electron- and photon-based fragmentation techniques is presented. A deep learning-based model trained to...

nature.com
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Shabaz Lab retweeted
Nature Methods@naturemethods
Mar 23
A mass spectrometry platform integrates alternative fragmentation techniques into standard LC-MS workflows for broader community adoption. nature.com/articles/s41592-0…
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🔬 New research from Oxford Chemistry advances proteomics by combining multiple fragmentation strategies within a single mass spectrometry platform. Read more: chem.ox.ac.uk/article/combin…

Combining fragmentation strategies for next-generation proteomics

chem.ox.ac.uk
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I am pleased to announce the final program for the EUPA Brixen Proteomics Summer School 2026 now also a @FEBSJournal Advanced Lecture Course Thank you @thermofisher @EvosepBio Ionopticks @ReSyn_Bio @SIGNATOPE_GmbH Momentum Biotechnologies @bsi_peaks @biognosys for support!
www.brixenproteomics.org

ALT www.brixenproteomics.org
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Turns out you can do cysteine profiling without lysing the cells first. nature.com/articles/s41557-0…

Biocompatible ligand balancing in transition metal coordination enables benign in-cell protein...

Nature Chemistry - The perceived toxicity of organometallic reagents has limited their use in living systems. Now it has been shown that balancing flexible chelation with biocompatible ligands...

nature.com
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The Brixen Proteomics Summer School is back! Speaker list is already looking strong!
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We asked the question do solid particles have a role to play in the separation space within modern proteomics? Would these very small particles in short columns for fast gradients be a good idea for modern fast and sensitive MS? The answer was not no! pubs.acs.org/doi/10.1021/acs…

Are Solid Particles Ready for Prime-Time Proteomics?

Abstract. We evaluate the performance of nonporous C-18 stationary phases in high-speed proteomics workflows. We employed two commercially available sub-2

pubs.acs.org
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Proud to announce my first publication is out in @PLOSPathogens ! Huge thanks to my co-first author Wael, @brennanlab, and @castello_lab. The paper explores how tick RNA-binding proteins respond to UUKV infection, you can find the paper here dx.plos.org/10.1371/journal.…
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🚨 New paper in @PLOSPathogens! 🚨 Together with @brennanlab, we mapped – for the first time – how tick RNA-binding proteins rewire during virus infection. 283 proteins changed activity, revealing key players in viral fitness. Read here: doi.org/10.1371/journal.ppat…

Uukuniemi virus infection causes a pervasive remodelling of the RNA-binding proteome in tick cells

Author summary In host cells, RNA-binding proteins play essential roles in the life cycle of many viruses by influencing how viral RNA is processed, translated, replicated and assembled into virions....

journals.plos.org
Alexandra Wilson@Wilsonasaur
13 Aug 2025
Proud to announce my first publication is out in @PLOSPathogens ! Huge thanks to my co-first author Wael, @brennanlab, and @castello_lab. The paper explores how tick RNA-binding proteins respond to UUKV infection, you can find the paper here dx.plos.org/10.1371/journal.…
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We were just using NHS chemistry for some protein conjugation when we noticed some odd prominent side products. It turns out thio-nhs reagents (a major class of reagents) has some notable side reactions that happen all the time. nature.com/articles/s41467-0…

Thio-NHS esters are non-innocent protein acylating reagents

Nature Communications - N-Hydroxysuccinimide (NHS)-ester derivatives are one of the most widely used acylating agents. In this work, the authors report that ring-opening reaction of the succinimide...

nature.com
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So Nikita really wanted to present his poster (ThP648) on the omnitrap at #ASMS2025. Well with the power of the internet he can! Visa be damned. Also he is fresh and not destroyed by all the vendor hospitality...
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Here's an LC story from our lab. Unlike a lot of stories with questions as titles this one is a maybe yes! Interesting for those high throughput proteomics people! biorxiv.org/content/10.1101/…

Are Solid Particles Ready for Prime-Time Proteomics?

We evaluate the performance of nonporous C-18 stationary phases in high-speed proteomics workflows. We employed two commercially available sub-2 µm nonporous particle (NPP) materials, ODS-IIIE (1.5...

biorxiv.org
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Deadline is fast approaching!
Shabaz Lab@ShabazLab
11 Mar 2025
The Brixen summer school speaker list has been finalised. It's going to be another fantastic edition!
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🚨🚨News: Interested in the proteome configuration that makes a cell permissive or hostile to virus infection? Why mRNA levels do not fully reflect the proteome status after interferon treatment? See the new paper from @castello_lab and Mohammed lab mcponline.org/article/S1535-…
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We are excited to share our new Nature Methods paper describing the Chip-Tip workflow for single-cell proteomics identifying >5,000 proteins in single cells, enabling PTM analysis without enrichment and throughput of up to 120 single cell samples per day: rdcu.be/d6qJe
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