Lab studying evolution of proteins and viruses. Affiliated with @fredhutch @HHMINEWS @uwgenome. Opinions are my own and do not reflect those of my employer.
Seattle, WA
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We have measured 47,851 neutralization titers of current human sera vs current influenza strains to inform vaccine update & analyses of viral evolution.
New H1N1 strains (eg, D.3.1.1 + G155E) have reduced neutralization, as do some H3N2 subclade K descendants.
biorxiv.org/content/10.64898…
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This is third installment of our efforts to generate near real-time data on human neutralizing antibody landscape to influenza. Our hope is that these data can improve vaccine-strain selection and ability to forecast short-term evolution of human seasonal influenza.
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The work was led by @CKikawa , @aw_butler, and @huddlej.
Thanks also to S Turner, H Peck, J Englund, K Lacombe, M Busch, M Lanteri, M Stone, B Spencer, @GreningerLab, D Smith, S Wallace, H Marshall, S Tosif, @SCOTTeHENSLEY, & Ian Barr.
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Here is a link to slides I will present today at Options XIII Conference for Control of Influenza meeting: slides.com/jbloom/options202…
Slides describe using sequencing-based neutralization assays to characterize human neutralizing antibody landscape to seasonal influenza virus in near real time.
Measurements are informative for vaccine strain choice.
Our latest data from last few weeks identifies emerging variants of seasonal H3N2 & H1N1 to which current human sera has reduced titers. These include H3N2 subclade K variants w V223I, and H1N1 subclade D.3.1.1 variants including w G155E.
All raw data along with interactive visualizations are available at links in the slides. The section on our latest unpublished data starts on slide 19.
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In new study led by Bernadeta Dadonaite, we show many influenza HAs (H5, H7, H9, H1, H2, H3) can use avian or human MHC-II to enter cells.
We then use novel combo of deep mutational scanning & cryoEM to define how H5 HA binds tufted duck MHC-II
Preprint: doi.org/10.64898/2026.07.17.…
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Note our study used pseudoviruses and conditionally replicative virions to ensure biosafety, and reports deep mutational scanning only for HA usage of tufted duck MHC-II to limit any information hazard concerns.
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Please see the preprint for additional details: doi.org/10.64898/2026.07.17.…
Thanks to Bernadeta Dadonaite for leading study, and @anniedosey, @Jenny_Ahn0, @timcyuu, Sara Sunshine, Ariana Farrell, and @KingLabIPD for valuable contributions.
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