If you use an NES-fused Cas13 protein or want to focus on cytosolic events such as translation, use the U1 promoter rather than the U6 promoter for crRNA expression. By simply changing the promoter, you will achieve much higher efficiency. 2/n
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I would like to thank @hkusbms and @hkumed for their support in fostering a research-oriented environment! 3/3
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All the plasmids have been deposited at @Addgene. addgene.org/browse/article/2…
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