Shih-Chieh (Jeff) Ti@JeffTiLab

Assistant Professor @ University of Hong Kong, School of Biomedical Sciences

 Hong Kong
tilab.org
Joined January 2018
5 Photos and videos
A new research article from the TI Lab. rdcu.be/fdx4v In this article, we reveal the molecular mechanism by which a specific tubulin isotype renders cancer cells resistant to paclitaxel, a WHO-listed essential medicine for cancer treatment.
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Shih-Chieh (Jeff) Ti retweeted
Chul KWON@schul_kwon
Aug 13
Excited to share our latest work on high-resolution comparative RNA knockdown profiles of shRNA, CasRx, and PspCas13b systems! biorxiv.org/content/10.1101/…
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Alushin_Lab@Alushin_Lab
Apr 22
Very pleased that after a rigorous (and lengthy) review process, our paper visualizing how myosin generated forces modulate actin filament structure for mechanosensitive recogntion by α-catenin has appeared in @Nature nature.com/articles/s41586-0…

Myosin forces remodel F-actin for mechanosensitive protein recognition

Nature - Structural studies demonstrate that myosin generates forces that cause structural changes in actin, modulating cooperative binding by α-catenin and thus signal transduction and...

nature.com
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Shih-Chieh (Jeff) Ti retweeted
Alushin_Lab@Alushin_Lab
Mar 9
Very excited to share our latest preprint, where we visualize the impact of force on the catch bonding actin crosslinker α-actinin-4. Led by @alfredcchin, a great collaboration with Glen Hocky's lab. biorxiv.org/content/10.64898…
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RT @Zhang_Suyang: Two weeks left to apply for a PhD with us at MRC-LMB in Cambridge. Join our team for an exciting project on transcription…
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nature.com/articles/s41567-0… The latest research article from the Ti Lab is finally out today in Nature Physics.

Tubulin isotypes of C. elegans harness the mechanosensitivity of the lattice for microtubule...

Nature Physics - Proteins interact with both the exterior and interior of microtubules. Here the relationship between microtubule building blocks and the accessibility of the microtubule interior...

nature.com
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regulate the accessibility of the confined microtubule lumen remain elusive. In this paper, by employing optical tweezers, cryo-EM, computer modeling, and single-molecule fluorescence microscopy, we found that tubulin isotypes can regulate how microtubules respond to forces,
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which then determine the accessibility of the lumen to an luminal enzyme. This study opens new avenues to understanding the roles of microtubule building blocks in diverse biologically relevant mechano-environments.
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Shekhar lab@sshekhr
26 Apr 2025
🎉🎉We’re on the cover of @NaturePhysics!! Here is the original paper nature.com/articles/s41567-0…
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NatureStructMolBiol@NatureSMB
22 Apr 2025
New online! The mechanochemical cycle of reactive full-length human dynein 1 bit.ly/3EIW4ku
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Chul KWON@schul_kwon
26 Feb 2025
Cytosolic CRISPR RNAs for efficient application of RNA-targeting CRISPR-Cas systems | EMBO reports embopress.org/doi/full/10.10… Our paper is out in @emboreports! 1/n

Cytosolic CRISPR RNAs for efficient application of RNA-targeting CRISPR-Cas systems

EMBO Reports - Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas) technologies have evolved rapidly over the past decade with the continuous discovery...

link.springer.com
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DivIDE conference@DivIDE_2025
18 Nov 2024
We are delighted to announce that the 3rd DivIDE Conference will take place from 22–25 September 2025 with a fantastic lineup of speakers. Registration opens soon - stay tuned and help us spread the word! We are looking forward to meet many of you in Barcelona! ☀️
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Alushin_Lab@Alushin_Lab
21 Jan 2025
Excited that the peer-reviewed version of our fascin structure paper is now out in @NatureSMB. Thanks very much to the referees for their constructive feedback. rdcu.be/d6Tct
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With help from @RuiZhangWUSTL we have successfully adopted Rui's protocol to achieve high-resolution cryo-EM 3D reconstruction of undecorated microtubules polymerized from recombinant tubulin. Please see the following user-friendly version of the workflow. drive.google.com/file/d/1WIE…

seam-search_final_Nov14_2024.html

drive.google.com
Rui Zhang@RuiZhangWUSTL
8 Nov 2024
I want to take the opportunity to mention that I've written a slightly more user-friendly tutorial for the original microtubule seam-search protocol, together with other useful MT processing tricks. evernote.com/shard/s178/sh/d…
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Shih-Chieh (Jeff) Ti retweeted
Cell Biology J-Club@cell_club
14 Nov 2024
Force-transducing molecular ensembles at growing microtubule tips control mitotic spindle size nature.com/articles/s41467-0…
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rdcu.be/dY5rl The latest research article from Ti Lab. We employed cryo-EM structural analysis, single-particle fluorescence microscopy, and recombinant tubulin to reveal how tubulin acetyltransferases modify the K40 residue inside the microtubule lumen. (1/N)
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processivity. Using recombinant tubulins that mimic preacetylated and postacetylated K40, we revealed the crosstalk between microtubule acetylation states and the cofactor acetyl-CoA in enzyme turnover. (3/N)
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This work is a great collaboration with Dr. Yuanliang Zhai , @ChaoguZheng, and @XiangDavidLi1 at the University of Hong Kong. (4/4)
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