Assistant Professor @ University of Hong Kong, School of Biomedical Sciences
Hong Kong
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A new research article from the TI Lab.
rdcu.be/fdx4v
In this article, we reveal the molecular mechanism by which a specific tubulin isotype renders cancer cells resistant to paclitaxel, a WHO-listed essential medicine for cancer treatment.
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which then determine the accessibility of the lumen to an luminal enzyme. This study opens new avenues to understanding the roles of microtubule building blocks in diverse biologically relevant mechano-environments.
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nature.com/articles/s41567-0…
The latest research article from the Ti Lab is finally out today in Nature Physics.
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With help from @RuiZhangWUSTL we have successfully adopted Rui's protocol to achieve high-resolution cryo-EM 3D reconstruction of undecorated microtubules polymerized from recombinant tubulin. Please see the following user-friendly version of the workflow.
drive.google.com/file/d/1WIE…
I want to take the opportunity to mention that I've written a slightly more user-friendly tutorial for the original microtubule seam-search protocol, together with other useful MT processing tricks.
evernote.com/shard/s178/sh/d…
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processivity. Using recombinant tubulins that mimic preacetylated and postacetylated K40, we revealed the crosstalk between microtubule acetylation states and the cofactor acetyl-CoA in enzyme turnover. (3/N)
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We found that one tubulin acetyltransferase molecule spans the luminal lattice, with the catalytic core docking onto α-tubulins and the C-terminal domain anchoring in taxane-binding pockets of β-tubulins. The presence of Paclitaxel inhibits luminal accessibility and enzyme (2/N)
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rdcu.be/dY5rl
The latest research article from Ti Lab. We employed cryo-EM structural analysis, single-particle fluorescence microscopy, and recombinant tubulin to reveal how tubulin acetyltransferases modify the K40 residue inside the microtubule lumen. (1/N)
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The last day of my first PhD student, @FGuoling , in the Ti Lab. Wish you all the best at MRC.
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Thank @Mill_lab for inviting us to contribute to this beautiful paper. It is cool to support this story with in vitro reconstituted dynamic microtubules using recombinant TUBA1A-TUBB4B tubulin.
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Having a Pollard lab meeting in the 2023 ASCB/EMBO meeting. It’s a great pleasure to meet the Pollard lab members across generations.
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I will be at B111 this afternoon. Feel free to stop by for a chat on tubulin. 😁 @ASCBiology
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Congratulations! Tarun 🎉@KapoorLab_RU
Congratulations to Professor Tarun Kapoor, Rockefeller University, on being named a 2023 ASCB Fellow!
ascb.org/society-news/ninete…
@RockefellerUniv
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We enjoyed the discussion with your group! Thanks for all the feedback and insightful questions. 😀
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cell.com/cell-reports/fullte…
The first research article of TiLab is out.😃 By employing the recombinant tubulin strategy, we reveal the roles of tubulin isotypes in regulating the activities of enzymes for tubulin tyrosination and microtubule stability.
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Please spread the word. Our core facility is recruiting at all different levels.
Come and join us! Scan the QR code to apply if you are interested.
#cpos #hkumed #sassoonroad #hiringnow #sciencejobopportinities
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Our first article! A mini-review on @FrontCellDevBio
Reconstituting Microtubules: A Decades-Long Effort From Building Block Identification to the Generation of Recombinant α/β-Tubulin frontiersin.org/article/10.3…
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