We are the Dewar lab at Vanderbilt University. We study the biochemistry of DNA replication using in vitro cell-free approaches. #GelsNotCells #SometimesCells
Nashville, TN
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Our first foray into replication fork processing is now available! 🐸🧍🧬🧪 Key points:
- Uncoupling causes degradation and reversal
- Degradation occurs before and after reversal
- Replisomes are retained throughout
See model for how we think this works🙏
nature.com/articles/s41594-0…
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Dewar Lab retweeted
Happy to share our study about the role of Top2 complexes in different chromatin contexts!
We find that Top2 catalytic inhibition leads exclusively to instability of the repetitive genome which depends on Top2a. For more details of how, please read:
biorxiv.org/content/10.1101/…
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The Cimprich lab has research assistant positions available. If you (or someone you know) might be interested please apply here:
careersearch.stanford.edu/jo…
careersearch.stanford.edu/jo…
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We're looking for a new research technician to join our lab! Reasons to join:
1. Great science 🐸🧬
2. Fun & supportive environment 😃🙌
3. Excellent training record 🥇🎉
tinyurl.com/DewarLabRA23
Please share with any class of '23 graduates who might be interested!
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AP endonuclease 2 (APE2) as an emerging therapeutic target– More from our special collection on DNA repair and cancer therapeutics. From @ShanYanlab @UNCCBiology academic.oup.com/narcancer/a…
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Our latest termination paper is online! 🐸🧬➡️⬅️🧬Key points:
- RTEL1-MCM10 is a 'backup' mechanism for when TOP2 is compromised
- MCM10 promotes RTEL1 binding/function
- RTEL1-MCM10 don't regulate topology but do act as a general stalled fork response
cell.com/cell-reports/fullte…
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Please forward this to any senior graduate students you know. Should be lots of fun! I can't wait to meet the participating trainees and hear about their research!
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🧬🛠️ We had an amazing time at the Mammalian DNA Repair GRC in Ventura, CA. Fantastic presentations and discussions at a great location. 🙏 to @Opreskolab and @RogerRogergr for organizing. Can't wait for the next one! @GordonConf #DNARepair
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Dewar Lab retweeted
Researchers from the @Topo_Lab were instrumental in the development of the first new antibiotic treatment for uncomplicated urinary tract infections in more than 20 years.
Their collaborators @GSK will soon be filing for FDA approval.
news.vanderbilt.edu/2023/01/…
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Check out our latest story in @MolecularCell. Our team pairs the dTAG system with deep genomic analysis to determine the spatiotemporal role of SMARCA5 in the maintenance of chromatin structure and function.
bit.ly/3GNCalx
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Postdoctoral positions available in Nussenzweig lab to study mechanisms involved in DNA repair- 1st position (wet lab) focuses on using cutting-edge cellular, molecular, and genomic tools. 2nd uses computational approaches to study DNA repair.
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Our new cryo-EM structure explains how the MRN complex can act both as DNA double-strand break sensor & DNA tethering device, read the paper here:
authors.elsevier.com/c/1gK10…
Huge thanks to all collaboration partners W.Roos, B.Steigenberger and C. Junge!
#cryoEM, #DNArepair, #MRN
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The Department of Biochemistry @VandyBiochem in Vanderbilt Basic Sciences @VUBasicSciences is recruiting new faculty! Apply here: apply.interfolio.com/112443
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📢 Hot off the press 📢
🧬 Ever wonder why some DNA sequences are difficult to replicate?
Using reconstituted replisomes we revealed that structure-forming repeats induce robust replication stalling and uncovered structure-specific recovery mechanisms:
nature.com/articles/s41467-0…
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Excited to share our latest paper, just published in EMBO J: embopress.org/doi/abs/10.152…
We found that topoisomerase II poisons exert different and unexpected effects on DNA replication. Congrats @sabrinadoessci1! Thanks also to our collaborators @Topo_Lab and @davidkcortez
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Dewar Lab retweeted
Really excited to share the methods chapter we wrote for DNA Damage Responses as a part of Methods in Molecular Biology series. Here we describe really fun assays to monitor DNA replication termination using 🐸🥚 extracts! #gelsnotcells @Dewar_Lab link.springer.com/protocol/1…
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Thrilled to announce another preprint from our lab! @LillianVCampos found that RTEL1 and MCM10 help replication forks overcome topological stress during termination. Thanks also to @BFEichman @DarrenHeintzman @TKavlashvili
RTEL1 and MCM10 overcome topological stress during vertebrate replication termination biorxiv.org/cgi/content/shor… #bioRxiv
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Dewar Lab retweeted
Not long left to apply for postdoc positions on 🧬 replication in my new lab @mrc_hgu @EdinUni_IGC. A range of projects available, some of them related to nature.com/articles/s41586-0…. Informal enquiries welcome.
elxw.fa.em3.oraclecloud.com/…
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A great opportunity for anyone looking for an exciting post-doctoral position!
Postdoc positions! Come join us and address key biological questions about how Replication Gaps impact cancer, therapy response and developmental disease while learning the latest genome analysis techniques.
Interested, Sharon.Cantor@umassmed.edu
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